Isolation, Screening, Charactization of Azospirillum spp from Rhisopheric Soil and Checking its Plant Growth Promoting Ability

Select Volume / Issue:

• Volume 13,Issue 2, Mar. 2026

Year:

2026

Type of Publication:

Article

Keywords:

Soil Microbial Communities, Plant Growth Promoting Rhizobacteria, Azospirillum spp, Rhizosphere, Indole-3-Acetic Acid, Phosphate Solubilization, Ammonia Production, Hydrogen Cyanide, Cellulase Activity, Wheat Seed Germination, Bioinocu

Authors:

Sengupta, Ms. Kshitija Manoj; Raut, Prof (Dr. ) S. V.; Pinto, Mrs. Lavina

Journal:

IJRAS

Volume:

13

Number:

2

Pages:

23-34

Month:

March

ISSN:

2348-3997

Abstract:

The present study was conducted to isolate, screen, and characterize plant growth promoting Azospirillum spp. from rhizospheric soil and evaluate their plant growth promoting (PGP) traits and their effect on seed germination and early plant growth under laboratory conditions. Rhizospheric soil samples were collected and Azospirillum spp. were isolated using selective media. The isolates were screened and characterized based on morphological and biochemical characteristics. The selected isolates were evaluated for plant growth promoting traits including Indole-3-acetic acid (IAA) production, phosphate solubilization, ammonia production, hydrogen cyanide (HCN) production, and cellulase activity using standard qualitative and quantitative methods. The effect of the isolates on seed germination and early plant growth was also evaluated under laboratory conditions. Three isolates from soil sample (S1, S2 and S3) were obtained and all isolates showed multiple plant growth promoting traits. IAA production ranged from 33.71-42.37 ppm, with isolate S1 showing the highest production. Phosphate solubilization ranged from 2.11-3.66 µg/ml, with S3 showing the highest activity and significant difference among isolates. Ammonia production and HCN production were positive in all isolates, and cellulase activity was also observed, indicating their multifunctional plant growth promoting potential. Although the quantitative values were moderate, the isolates showed consistent plant growth promoting traits. Seed germination and early plant growth studies indicated improved growth parameters in treated seeds compared to control. In conclusion, the isolated Azospirillum spp. demonstrated multiple plant growth promoting traits and showed potential for use as biofertilizers in sustainable agriculture due to their combined plant growth promoting activities.

Full text: IJRAS_1198_FINAL.pdf