Molecular Identification of Bacteria Spot of Tomato Incited by Xanthomonas campestris pv. vesicatoria, from Fields in the Ashanti, Brong Ahafo and the Upper East Regions of Ghana

Select Volume / Issue:

• Volume 2 Issue 2, March, 2015

Year:

2015

Type of Publication:

Article

Keywords:

Bacteria Spot, PCR, Xanthomonas campestris

Authors:

Lamini, S.; Cornelius, E. W.; Nutsugah, S. K.; Coffie, S. Aseidu-; Miller, S.; Gilbertson, R.

Journal:

IJRAS

Volume:

2

Number:

2

Pages:

103-107

Month:

March

Note:

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. CC BY-NC-SA 4.0 Creative Commons License: https://creativecommons.org/licenses/by-nc-sa/4.0/

Abstract:

Recently, tomato farmers in some areas in the Ashanti, Brong Ahafo and the Upper East Regions of Ghana reported the occurrence of a strange disease affecting their crop. Symptomatic plant samples were collected from some infected fields in the affected areas for proper identification of the causal organism. Preliminary test on isolates using potassium hydroxide (KOH) and Gram reaction revealed the causal organism to be gram negative bacterium belonging to the genus Xanthomonas.Xanthomonas genus and species specific primers were used in a polymerase chain reaction (PCR) assay to confirm the genus and identify the species asXanthomonas campestris pv. vesicatoria.

Full text: IJRAS_243_Final.pdf [Bibtex]